Figure 6. AGP-activated medium induces mitochondrial network collapse in cancer cells.
A375 cells in FBS/DMEM were plated in an 8-well chambered coverglass and treated with undiluted or 3-fold diluted AGP-activated medium for 24 h (A) or undiluted AGP-activated medium for the indicated times (B) or control medium for 24 h (C) at 37°C. After washing, the cells were stained with MitoTracker Red CMXRos for 1 h, and washed again. Images were obtained and analyzed using an EVOS FL Cell Imaging System at × 1200 magnification.