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. 2016 Feb 24;7(15):19997–20015. doi: 10.18632/oncotarget.7671

Figure 10. mTOR or PI3K inhibitor reduces the protective influence of fibroblasts in the co-cultures.

Figure 10

Melanoma cells were grown as mono-cultures or co-cultures with fibroblasts and treated with 0.5 μM BRAFi (A, B), 5 nM mTORi (A), 10 μM PI3Ki (B) or the combination as indicated (A, B). The level of pS6 in the co-cultures was analyzed after 24 h by flow cytometry (histogram inserts). Melanoma cell survival/growth was scored after 72 h by measuring bioluminescence and relating the signal in the treated cells to the signal in the non-treated controls (set to 100); average ± SEM (n=3). *, p<0.05 (unpaired t-test).