Figure 12. Treatment effect on lung metastases in vivo.

Melmet 5 cells were injected into mice i.v. and were allowed to grow for 6 days before initiation of the treatment with a vehicle (control), 50 mg/kg BRAFi, 5 mg/kg mTORi or a combination of both drugs every weekday until day 17, when the treatment was stopped. Tumor burden was scored at days 6 (start), 17 (finish) and 38 (post-treatment) by live imaging in vivo, measuring bioluminescence (p/s/cm²/sr) in the IVIS Spectrum instrument. A. The bioluminescence signal at days 17 and 38 was normalized to the signal at day 6 (for the non-normalized data, see Supplementary Figure S7). The number of animals per group is indicated in the legend. *, p<0.05 (unpaired t-test). B. Representative IVIS pictures showing the animals at the start and the end of the treatment.