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. 2016 Mar 3;7(15):20041–20053. doi: 10.18632/oncotarget.7884

Figure 4. Effect of miR-423 on the sensitivity of A549 and H1299 cells to cisplatin.

Figure 4

Effects of miR-432 inhibitor transfection on the cisplatin sensitivity were assayed by MTS method in A549 (A) and H1299 (B). Inhibitor control + Cisplatin vs miR-432 inhibitor + Cisplatin:*p < 0.05; **p < 0.01. The cell vitality (C) and apoptotic fraction (D) were analyzed in A549 and H1299 cells after cisplatin treatment at various concentrations with or without miR-432 mimics transfection. Cisplatin + mimics control vs Cispliatin + miR-432 mimics: *p < 0.05; **p < 0.01. Effects of cisplatin treatment at different concentrations and various time of on miR-432 expression in A549 (E) and H1299 (F). The expression of E2F3 and AXL mRNA was detected by RT-qPCR in A549 (G) and H1299 (H) after cisplatin (5 μg/ml) treatment. Western blotting was performed to analyse the expression of AXL (upper) and E2F3 (lower) in A549 (I) and H1299 (J) as indicated treatments. *p < 0.05.