Figure 3. Stromal cells show differential angiogenesis supporting function.

Monolayers of stromal cells were seeded into 24 well glass-bottomed imaging plates, and were pre-treated as specified, for three days to allow differentiation to myofibroblasts. Endothelial cells (2×10⁴ cell/well in 500μl) were added in a drop-wise and scattered fashion to each well. After 4 days, the cells were fixed and stained for CD31 and DAPI. A series of images was taken in a 4 × 3 grid using a 20x objective, for each duplicate treatment. One composite image is shown for each treatment for the WCB949 patient as a representative example (Scale bar=500μm) A. For each image composite, the surface area occupied by each CD31-positive structure was measured. Representative examples of areas taken for such measurements are shown B. The CD31-positive area is shown for all treatments in all 6 patients C. (*p<0.05, **p<0.01, ***p<0.001, Kruskal-Wallis test with Dunn's multiple comparison post test).