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. 2016 Mar 7;7(15):20312–20323. doi: 10.18632/oncotarget.7950

Figure 2. HBV envelope proteins localization and ER stress markers expression.

Figure 2

Immunofluorescence analysis of LHBs and MHBs proteins co-localization with the ER specific dye ER-Tracker in Huh-7 (A) and HepG2 (B) cells after transfection with Empty vector, pSVL or pSVM plasmids, or treatment with 10 nM TG for 72 hours. Immunofluorescence analysis has been performed under identical settings. Nuclei were stained with Hoechst 33342. Magnification is 630× and scale bar represents 5 μm. RT-qPCR analysis of BiP and CHOP after 72 hours transfection with Empty vector, pSVL or pSVM plasmids, or treatment with 10 nM TG in Huh-7 (C) and HepG2 (D) cells. mRNA expression was normalized to GAPDH and results are expressed relative to Empty vector for pSVL or pSVM plasmids transfected cells, and to untreated controls for TG treated cells, and set in both cases at 1.0. Shown are means ± SEM of three independent experiments performed in triplicates. *p < 0.05 vs. Empty vector.