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. 2016 Mar 7;7(15):20312–20323. doi: 10.18632/oncotarget.7950

Figure 4. Role of CB1 in ER stress induction.

Figure 4

(A) RT-qPCR analysis of CB1 expression after 24, 48 72 and 96 hours transfection with Empty vector, pSVL or pSVM plasmids, or treatment with 10 nM TG in Huh-7 cells. mRNA expression was normalized to GAPDH and results are expressed relative to Empty vector for pSVL and pSVM plasmids transfected cells, and to untreated controls for TG treated cells and both set at 1.0. Shown are means ± SEM of three independent experiments performed in triplicates. *p < 0.05 vs. Empty vector. BiP and CHOP expression has been analyzed in Huh-7 cells (B) after 72 hours transfection with Empty vector, pSVL or pSVM plasmids and/or treatment with the cannabinoid receptor antagonist AM251. mRNA expression was normalized to GAPDH and results are expressed relative to Empty vector for pSVL and pSVM plasmids transfected cells, and to untreated controls for TG treated cells and both set at 1.0. Shown are means ± SEM of three independent experiments performed in triplicates. *p < 0.05 vs. control, #p < 0.05 vs. pSVL or pSVM transfected cells. (C) RT-qPCR analysis of CB1 expression after 72 hours treatment with 10 nM AM251 and 10 nM TG in PLC/PRF/5 and HLF cells. (D) BiP transcript was detected in PLC/PRF/5 and HLF cells after 72 hours treatment with 10 nM AM251 and 10 nM TG. mRNA expression was normalized to GAPDH and results are expressed relative to untreated set at 1.0. Shown are means ± SEM of three independent experiments performed in triplicates. *p < 0.05 vs. control.