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. 2016 Mar 5;7(15):20338–20356. doi: 10.18632/oncotarget.7934

Figure 1. Everolimus suppressed cell proliferation and colony formation.

Figure 1

The ovarian cancer cell lines, HEY, SKOV3, OVCAR5, IGROV1 and OV433, were cultured for 24 h and then treated with varying concentration of everolimus (from 10 to 25000 nM) in 96 well plates for 72 h. Cell proliferation was assessed by MTT assay (A). The effect of everolimus on long term growth in SKOV3 and OVCAR5 was assessed through a colony-forming assay (B). Ten primary cultures of human ovarian cancers were cultured for 24 h and treated with everolimus at 10 to 500 nM for 72 h. MTT showed that everolimus decreased cell proliferation in primary cultures of ovarian cancer (C). *p < 0.05, **p < 0.01.