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. 2016 Mar 5;7(15):20338–20356. doi: 10.18632/oncotarget.7934

Figure 3. Everolimus induced cell oxidative stress in ovarian cancer cells.

Figure 3

The SKOV3 and OVCAR5 cells were cultured for 24 h and treated with everolimus at 10 to 500 nM doses for 12 h. ROS production was tested using the DCFH-DA assay. Everolimus increased the ROS level in a dose-dependent manner in the SKOV3 and OVCAR5 cells after 12 h of treatment (A). The cells were treated with various concentrations of everolimus for 6 h. Western blotting showed that everolimus increased the expression of stress proteins (PERK, BIP and CALNEXIN) in the SKOV3 and OVCAR5 cell lines (B). *p < 0.05.