Skip to main content
. 2016 Feb 27;7(15):20368–20380. doi: 10.18632/oncotarget.7783

Figure 4. Effects of calgranulin B internalization on colon cancer cell lines.

Figure 4

A. MTT assay results showed increased cell death in SNU-C4 cancer cells 48–72 h after calgranulin B treatment compared to SNU-81 and HCT-116 cells. B. FACS analysis confirmed cell cycle changes, most significantly arrest at sub-G1 phase, in all tested cell lines (excluding HeLa) at 72 h post calgranulin B treatment (100 nM). C. TUNEL assay showed that apoptosis was effectively increased in colon cancer cell lines at 72 h post treatment. D. At 72 h post calgranulin B (100 nM) treatment, intracellular signaling was assessed using western blot analysis. Total levels of cleaved caspase-3 and p53, as well as phosphorylated AKT, ERK and JNK, were increased after treatment. In contrast, β-catenin and E-cadherin levels were suppressed in colon cancer cells.