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. 2016 Feb 27;7(15):20368–20380. doi: 10.18632/oncotarget.7783

Figure 5. Decreased aurora A kinase activity upon calgranulin B binding.

Figure 5

A. Recombinant human calgranulin B V5-tagged vector construction. The recombinant protein was fused with GST at the N-terminus for purification and the V5 tag at the C-terminus for protein–protein interactions. B. SDS-PAGE gel showing the glutathione S-transferase (GST)–human calgranulin B fusion protein purified from E. coli. Lanes 1–4 indicate whole homogenate, GST column flow-through, GST column wash, and eluted fusion protein, respectively. C. In a human protein microarray, aurora A kinase was identified as a calgranulin B binding candidate. D. Immunoprecipitation (IP) of calgranulin B and aurora A kinase confirmed the binding. E. Calgranulin B binding suppressed aurora A kinase activity in a dose-dependent manner.