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. 2016 Mar 7;7(15):20381–20394. doi: 10.18632/oncotarget.7953

Figure 2. Overexpression of miR-148a suppresses 4T1 lung metastasis.

Figure 2

(A) Expression levels of miR-148a were determined in M-I, M-II, M-III, and M-IV cells and normalized to the expression level in M-II. Error Bars indicate Standard Errors (n = 3). (B) Expression levels of miR-148a were determined in 4TO7, 66c14, and 4T1 cells and normalized to the expression level in 4T1. Error Bars indicate Standard Errors (n = 3). (C) Expression levels of miR-148a were determined in 4T1 cells with overexpression of miR-148a (MiR-148a) normalized to control cells (VEC). Error Bars indicate Standard Errors (n = 3). (D–F) 4T1 cells with overexpression of miR-148a (miR-148a) and control cells (VEC) were examined for growth (D), viability (E), and migration/invasion (F). Error Bars indicate Standard Errors (n = 3). P values were calculated with two-way ANOVA (D) or unpaired, two-tailed t test (E). (G) Tumor sizes were measured and calculated at the indicated time points. P value was calculated with two-way ANOVA. Error Bars indicate Standard Errors (n = 5). (H) Representative pictures of lungs with metastatic nodules developed from 4T1-VEC or 4T1-miR-148a cells. (I) Lung nodules were quantified. Error Bars indicate Standard Errors (n = 5). P value was calculated with unpaired, two-tailed t test.