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. 2016 Mar 7;7(15):20381–20394. doi: 10.18632/oncotarget.7953

Figure 4. Overexpression of miR-148a regulates cancer cell lung colonization.

Figure 4

(A–F) The lung colonization ability of cancer cells with manipulated levels of miR-148a and control cells were determined by quantification of lung nodules raised from cancer cells administered directly into the blood. Error bars are standard errors (n = 5). P values were calculated with unpaired, two-tailed t test. (A) and (B) 4T1 cells with over expression of miR-148a and control cells. Representative picture (A) and quantification (B) are shown. (C) and (D) 4TO7 cells with down-regulation of miR-148a and control cells. Representative picture (C) and quantification (D) were shown. E and F, MDA-MB-231 cells with over expression of miR-148a and control cells. Representative picture (E) and quantification (F) are shown. (G) Analysis of cancer cell extravasation at day 1 and day 4 after administration. Cancer cells that underwent extravasation into lung were quantified by flow cytometry and normalized to 4T1 control cells. Error bars are standard errors (n = 3). P values were calculated with two-way ANOVA.