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. 2016 Mar 7;7(15):20381–20394. doi: 10.18632/oncotarget.7953

Figure 5. Identification of WNT1 and NRP1 as target genes of miR-148a in MDA-MB-231 cells.

Figure 5

(A) Illustration of strategy used to identify target genes of miR-148a. (B) qRT-PCR analysis was performed to validate mRNA levels altered by overexpression of miR-148a. (C) Protein abundance of WNT1 and NRP1 altered by miR-148a were determined by Western Blot. Two levels of miR-148a overexpression (150× and 20×) were achieved in MDA-MB-231 cells. (D) Correlation analysis with linear regression between levels of miR-148a and its target genes was done with GraphPad Prism. The expression levels were extracted from the TCGA database. (E) The 3′-UTR of the WNT1 and NRP1 genes contain binding sites for miR-148a seed region according to the TargetScan prediction program.