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. 2016 Mar 8;7(15):20532–20548. doi: 10.18632/oncotarget.7978

Figure 5. hnRNP A1 is involved in the regulation of miR-24 levels.

Figure 5

(A) HCT 116 and MCF-7 cells were treated with melatonin for 72 h and protein extracts were subjected to immunoblot with the indicated antibodies. Numbers indicate the densitometry ratio between hnRNP A1 and 20Sα5 signals. (B) HCT 116 cells were transfected with the indicated plasmids and treated with melatonin for 72 h, miR-24 levels were assessed by quantitative Real-Time PCR. *p < 0,01. (C) HCT 116 and MCF-7 were transfected with the indicated siRNAs. 72 h post transfection, cell lysates were subjected to immunoblot with the indicated antibodies. Numbers indicate the densitometry ratio between hnRNP A1 and tubulin or nucleolin signals, normalized to siGFP.