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. 2016 Mar 8;7(15):20561–20573. doi: 10.18632/oncotarget.7987

Figure 6. Inhibition of RalA by RalA siRNA or miR-181a mimic reduces the phosphorylative signal proteins.

Figure 6

Phosphorylative signal proteins in K562 cells transfected with RalA siRNA (A) or miR-181a (B) were comprehensively investigated using an antibody microarray system compared to control. The phosphorylation ratio (Phos Ratio) was used as the modulation difference of phosphorylation sites between two samples and was computed as follows: Reduction rate (%) = (Average value without RalA siRNA or miR-181a treatment–Average value with control treatment) / Average value with control treatment × 100. (C) Comparison of inhibitory spectrum of miR-181a and RalA siRNA results in the identification of phosphorylation targets that are associated with inhibitory effects of miR-181a and RalA siRNA in K562. Eight phosphorylation signal molecules were generally decreased by either miR-181a or RalA siRNA in K562 cells.