Skip to main content
. 2016 Mar 8;7(15):20561–20573. doi: 10.18632/oncotarget.7987

Figure 7. Western blot validation of the several key phosphorylation signal proteins through overexpression of RalA or RalA siRNA/miR-181a mimic.

Figure 7

Overexpression of RalA activation of Ras-related phosphorylative signaling pathway. Total cell lysate was isolated from K562 cells transfected with RalA plasmid, and analyzed for the expression levels of Phospho P38 MAPK (A), Phospho SAPK/JNK protein (B), and Phospho Akt/Erk (C). Inhibition of Ras-related phosphorylative signaling pathway by transfection with RalA siRNA or miR-181a. Total cell lysate was isolated from K562 cells transfected with RalA siRNA or miR-181a, and analyzed for the expression level of Phospho P38 MAPK (D), Phospho SAPK/JNK protein (E), and Phospho Akt/Erk (F).