Table I. Effect of IR on levels of PAF and ox-GPCs in B16F10 tumor cells.
| Glycerophosphocholine | Control Mean (SEM) | RT Mean (SEM) |
|---|---|---|
PAF 
|
5.2 (2.9) | 114(11) |
PAcPC 
|
48 (18) | 955(125) |
BPAF 
|
0.2 (0.2) | 9.6 (2.4) |
PBPC 
|
5.8 (3) | 211 (43) |
PPrPC 
|
0.9 (0.5) | 2.4 (0.9) |
PHPC 
|
0 (0) | 15.9 (7.1) |
AzPAF 
|
7.7 (5.6) | 3.2 (3.2) |
PAzPC 
|
235 (148) | 143 (70) |
OVPAF 
|
0 (0) | 0 (0) |
POVPC 
|
7.1 (2.0) | 5.4 (0.3) |
PONPC 
|
11.5 (4.2) | 11.7 (0.5) |
| Lyso-PAF | 54 (7) | 56 (16) |
B16F10 cells were irradiated with 10Gy or sham-irradiated in vitro. After 1 h of incubation, lipid extracts were analyzed by HPLC/MS/MS using deuterium-labeled internal standards to quantify PAF and Ox-GPC. Data are pg/106 cells from at least three separate experiments. The names of each ox-GPCs in this table are: PAF, platelet-activating factor; PAcPC, 1-palmitoyl-2-acetyl-GPC; BPAF, 1-O-hexadecyl-2-butanoyl-GPC; PBPC, 1-palmitoyl-2-butanoyl-GPC; PPrPC, 1-palmitoyl-2-propanoyl-GPC; PHPC, 1-palmitoyl-2-hexanoyl-GPC; AzPAF, 1-O-hexadecyl-2-azeleoyl-GPC; PAzPC, 1-palmitoyl-2- azeleoyl-GPC; OVPAF, 1-O-hexadecyl-2-(5)oxo-valeroyl-GPC; PONPC, 1-palmitoyl-2-oxononanoyl-GPC; Lyso-PAF, inactive precursor of PAF.