Figure 3. IL10-deficent mice failed to develop lung cancer induced by Kras4bG12D and EGFRL858R.

A. The mRNA level of IL10 in indicated transgenic mice. B. The protein level of IL10 was detected by IHC. C. After doxycycline administration for 5 months, lungs of Kras4b^G12D mice were excised for counting surface tumor nodules. D. Lungs excised from doxycycline-treated Kras4b^G12D (5 months) and EGFR^L858R (1 month) were immunohistochemically stained by HE and the anti-CD163 antibody, and immunofluorescently stained by anti-CD4 and anti-FoxP3 antibodies. Brown and grey in slides of Kras4b^G12D and EGFR^L858R, respectively, represent CD163⁺ cells.