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. 2016 Mar 3;7(15):20840–20854. doi: 10.18632/oncotarget.7894

Figure 4. IL10 regulates EGFR expression through Src activation.

Figure 4

A. The proliferation-related gene expression profile from microarray analysis in EGFRL858R and EGFRL858R/IL10−/−. B (a). Lung extracts were homogenized for detecting EGFR expression by Western blotting, and the signal of EGFR was quantified. (b). Data was expressed as mean±s.e.m. (***p<0.001). (c). The histological slides of lungs were stained for EGFR by IHC. C (a). Four scores to define IL10 expression detected by IHC in 192 lung cancer patients. (b). The IL10 expression in stages I&II and III&IV. (c). Negative (scores 0 and 1) and positive (scores 2 and 3) IL10 expression was correlated with survival period of patients. D. EGFR expression was detected in normal and tumor parts in 27 IL10-positive patients. (a). Upper panel is the images of Western blotting for EGFR. (b). Lower panel is the correlation of survival with IL10-positive/EGFR overexpression (IL10+EGFR) and IL10-positive only (IL10). E. Upper panel is the schedule of injection with the indicated drug. Lower panel: EGFRL858R mice were injected with gefitinib with or without anti-IL10 antibody injection for 4 weeks. Lungs were excised for HE staining.