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. 2016 Mar 10;7(15):20855–20868. doi: 10.18632/oncotarget.8028

Figure 6. The DNM-mediated membrane fission machinery regulates Atg9 vesicle generation at the Rab11-positive compartments.

Figure 6

Tamoxifen-inducible DNM triple KO (TKO) mouse fibroblasts stably expressing Atg9a-GFP were generated as described in the Materials and Methods. (A) Depletion of DNMs were analyzed by immunoblotting using the indicated antibodies. (B) The cells were pre-starved for 45 min and then starved in the presence of 120 μM Dynasore for 1 h. The fluorescence images were analyzed by confocal microscopy. Arrowheads represent Atg9 signals adjacent to the plasma membrane. (C) The cells were pre-starved for 45 min, starved in the presence of 120 μM Dynasore or control DMSO for 1 h, stained for Rab11, and analyzed by confocal microscopy. 3D images of the boxed areas are shown in the right panels. Arrows indicate Atg9- and Rab11-positive vesicle-like structures. Scale bars represent 10 μm.