Figure 1. Geminin promotes acetylation of chromatin-bound HMGB1.

(A) The levels of indicated proteins in naïve HME, iGem9 cells (defined as Dox-induced for 72 h) and the TNBC cell lines MDA-MB-231, MDA-MB-468 and BT549 sonicated extracts. (B) The levels of indicated proteins on the chromatin of iGem9 cells synchronized in G₂/M, M/G₁ and G₁/S phases. (C) IP experiments using geminin specific antibody on chromatin isolated from G₂/M, M/G₁ and G₁/S phase iGem9 cells and blotted for the indicated proteins. (D) IP experiments using geminin specific antibody on G₂/M phase iGem9 chromatin extracts of cells transfected with siLuc, siGem or siAbl for 72 h or treated with vehicle or 10 μM imatinib for 24 h. IP experiments using HMGB1 specific antibody (E) or CBP specific antibody (F) on iGem9 cells synchronized in G₂/M-phase sonicated extracts (first lanes), or chromatin extracts following 72 h of transfection with siLuc or siGem or 24 h treatment with vehicle or 10 μM imatinib. (G) Schematic representation of the data presented through out this figure. In all parts of the figure experiments were done between 2–3 separate times.