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. 2016 Mar 14;7(15):20919–20933. doi: 10.18632/oncotarget.8062

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Copyright: © 2016 Dowling et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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The effect of overexpressing PKC Beta II in colon cancer cells was examined using HCT116 cells stably over expressing HA_PKCBeta II. (A) Representative western blot showing the expression of p-PKC Beta II in stable overexpressing HCT116 cells (n = 3). (B) Representative images demonstrating the difference in the cells ability to form colonies when overexpressing PKC Beta II (n = 3). (C) Images demonstrating the difference in the size of cells growing in a 3D matrix over 144 hours (n = 3). Bar graph represents the percentage difference in the size of 3D cultures at 144 hours. Confocal images are cells harvested and stained after 144 hours (n = 3). (D) Representative image of cells that were scored with a wound and allowed to migrate over 24 hours. Bar graph represents the percentage wound closer after 24 hours (n = 3). (E) Representative graph of cells migrating over a period of 72 hours analysed in real time on the xCELLigence system (n = 3). Bar graph represents the percentage difference in cell index. (F) Representative graph of cells invading over a period of 72 hours analysed in real time on the xCELLigence system (n = 3). Bar graph represents the percentage difference in cell index.

The effect of overexpressing PKC Beta II in colon cancer cells was examined using HCT116 cells stably over expressing HA_PKCBeta II. (A) Representative western blot showing the expression of p-PKC Beta II in stable overexpressing HCT116 cells (n = 3). (B) Representative images demonstrating the difference in the cells ability to form colonies when overexpressing PKC Beta II (n = 3). (C) Images demonstrating the difference in the size of cells growing in a 3D matrix over 144 hours (n = 3). Bar graph represents the percentage difference in the size of 3D cultures at 144 hours. Confocal images are cells harvested and stained after 144 hours (n = 3). (D) Representative image of cells that were scored with a wound and allowed to migrate over 24 hours. Bar graph represents the percentage wound closer after 24 hours (n = 3). (E) Representative graph of cells migrating over a period of 72 hours analysed in real time on the xCELLigence system (n = 3). Bar graph represents the percentage difference in cell index. (F) Representative graph of cells invading over a period of 72 hours analysed in real time on the xCELLigence system (n = 3). Bar graph represents the percentage difference in cell index.