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. 2016 Mar 21;7(15):20999–21012. doi: 10.18632/oncotarget.8236

Figure 5. RUNX2 binds to with CXCR4 promoter.

Figure 5

(A) ChIP shows a candidate binding region (3F to 4R, 277 bp) of RUNX2 in CXCR4 promoter. (B) Luciferase activity assay shows that the predicted site (−1046 to −1032), but not mutants, is bound by RUNX2 in CXCR4 promoter. (C) Direct binding of RUNX2 to CXCR4 promoter region determined by EMSA. Lane 1 and 4 for nuclear extract binding reaction; lane 2 for negative control; lane 3 for competition test; lane 5 to 8 for supershift. **P < 0.01, Student's t test.