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Kinetics of hyaluronan (HA) suppression in blood upon in‐vivo treatment with 4‐methylumbelliferone (4‐MU). C57BL/6 mice were fed chow containing 5% 4‐MU or control chow ad libitum. Serum from both treatment groups was taken at baseline (day 0) and on days 2, 4 and 7, and HA levels were measured in triplicate using an HA‐specific enzyme‐linked immunosorbent assay (ELISA) assay. *P < 0·05, n = 3–5 mice per group.
