FIG. 5.

Mass spectrometric assessment of residual proteins following decellularization of wild-type versus α-gal KO pig lungs demonstrates overall concordance in residual proteins detected. Positively identified proteins in decellularized wild-type versus α-gal KO pig lungs (i.e., those proteins that exceeded the FDR cutoff for identification) from each method of decellularization were assigned to groups according to subcellular location (cytoskeletal, cytosolic, ECM, membrane-associated, nuclear, and secreted). Heatmaps were generated using the log normal transformation of total spectral counts for all positively identified proteins and grouped by category. Representative heatmaps from 6 wild-type and 10 α-gal KO pig lungs are shown. FDR, false discovery rates. Color images available online at www.liebertpub.com/tec