FIG. 6.

HBE, hMSC, and HLFs demonstrate comparable seeding patterns and viability for up to 28 days following inoculation into both wild-type and α-gal KO pig lungs, but CBF cells only survive for 7 days. Representative H&E low power (100 × ) photomicrographs show characteristic recellularization patterns 1 day postinoculation of each cell type (left column) and the last day viable cells were observed (right column) in acellular wild-type (A, B) or α-gal KO (C, D) pig lungs. Representative images from 33 wild-type and 17 α-gal KO lungs seeded with each cell type are shown. (B, D) High power (400 × ) images of the respective seedings into either acellular wild-type (B) or α-gal KO (D) pig lungs. In general, cells that do not interact with the ECM scaffold and remain in the airspaces or vascular spaces unattached to any matrix demonstrated rounding up of cells and nuclear fragmentation, consistent with anoikis or apoptosis. HBE, human bronchial epithelial; HLF, human lung fibroblasts; hMSC, human bone marrow-derived mesenchymal stromal cell. Color images available online at www.liebertpub.com/tec