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. 2016 Aug 1;14(6):345–354. doi: 10.1089/adt.2016.717

Fig. 1.

Fig. 1.

An Mtb infection model to generate large Mtb/macrophage aggregate structures. THP-1 monocytes were infected with Mtb-GFP at an MOI of 25 and Mtb/macrophage aggregate formation was documented. Representative bright-field and GFP-merged images of Mtb-infected THP-1 aggregate structures are shown (A) on day 4 and (B) day 10 postinfection. (C) Generation of Mtb/macrophage aggregate structures in 96-well plate format. Bright-field and GFP-merged images were acquired 7 days postinfection by automated imaging. Enlarged view of images from (D) well A6 with 2.5 × objective and (E) well B5 with 20 × objective are shown. MOI, multiplicity of infection.