Table 1.

Resazurin Assay Protocol

Step	Parameter	Value	Description
1	Titrate drugs	100 μL	Twofold serial dilutions in appropriate concentration range
2	Plate Mtb	100 μL	Broth-grown or Mtb/macrophage aggregates (6 million/mL)
3	Incubation time	5 days	37°C without shaking
4	Resazurin addition	20 μL	Final concentration: 100 μM resazurin and 0.5% Tween 80
5	Assay development	16–30 h	37°C without shaking
6	Assay readout	530/590 nm	Measure fluorescence on plate reader