FIG. 1.

Scaffold characterization: morphology and cell seeding. SEM micrographs of (A) semi3D-random scaffold; (B) semi3D-aligned scaffold; (C) semi3D-random scaffold coated with laminin; (D) semi3D-random scaffold coated with Cultrex. Scale bar: 10 μm. (E–P) Conventional fluorescence micrographs showing the SH-SY5Y cell deposition (labeled by β-III-tubulin) on the semi3D uncoated (E–J) and laminin-coated (K–P; laminin is in red) PLLA scaffolds, having random (E–G; K–M) and aligned (H–J; N–P) topography (E, H, K, N); HCS visualization of the same cell preparations (semi3D, uncoated, random: F,G; semi3D, uncoated, aligned: I,J; semi3D, laminin-coated, random: L,M; semi3D, laminin-coated, aligned: O,P). HCS, high-content analysis; PLLA, poly(L-lactic acid); SEM, scanning electron microscopy.