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. 2016 Aug 1;5(1):201–211. doi: 10.1089/biores.2016.0025

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© Vito Antonio Baldassarro et al. 2016; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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FIG. 4. — Effect of different scaffolds in the neural and astroglial specification of NSCs. (A, B) Representative images of plated NSCs at 7DIV grown on 2D (A) and semi3D scaffolds (B), stained with β-III-tubulin (green), GFAP (red) and Hoechst (blue). (C, D) The graphs indicate the percentage of GFAP (C) and β-III-tubulin (D)-positive cell, according to the PLLA topography and coating. Data are expressed as mean ± SD, and represent the mean of three independent experiments. Statistical analysis: two-way ANOVA (GFAP F[7, 32] = 3.404; β-III-tubulin F[7, 30] = 7.136) followed by Tukey's post test. Asterisks represent the difference between the semi3D group versus the relative 2D control group within the same DIV (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001).