Table 2.

FRDA iPSC Lines Reprogrammed from Patient Fibroblasts

Cell line	Mutation GAA1/GAA2	Reprogramming	No. of clones
68	570/1200	OSKM Retro	5
4491	559/G130V	OSKM Retro	3
GM03665	816/1400	OSKM Retro	3
GM04078	341/480	OSKM Retro	4
68_E	570/– and 1200/–	OSKM Retro	2
68	570/1200	OSKM Sendai	3
4259	404/920	OSKM Sendai	2
281	630/806	OSKM Sendai	3

Lines designated GM were derived using fibroblasts obtained from Coriell Cell Repositories. Line 68_E is a zinc finger-edited line with one GAA allele corrected by excision of the expanded sequence.¹⁵ Two different methods, retroviral and Sendai virus transduction, were used to establish these lines. All lines underwent rigorous testing for pluripotency, lineage differentiation, and karyotype analyses. As GAA repeats continuously expand in FRDA iPSCs, the repeat number is based on determination in fibroblasts prior to reprogramming.