FIG. 3.

Human breast cancer-derived soluble factors increased BM-MSC osteogenic differentiation. (A) 2D tumor-conditioned media was collected from MDA-MB231 cultured on tissue culture plastic for 24 h in low serum media, after which media was collected, concentrated, and reconstituted with the appropriate BM-MSC culture media. (B) After 21 days, 2D tumor-conditioned media (+tumor factors) did not alter BM-MSC growth patterns as assessed by fluorimetric DNA analysis. (C) Tumor-derived factors enhanced ALP enzyme activity following induction, indicating increased osteogenic differentiation. Same letters (a, b, or c) indicate similar mean values, and groupings with different letters indicate that the difference is significant by post hoc comparison of means with Bonferroni correction (p < 0.05). 2D, two dimensional. Color images available online at www.liebertpub.com/tea