Fig. 3.

α4β2 agonist (A) and antagonist (B) assay evaluations. Concentration–response curves were obtained for six reference agonists and three inhibitors in two independent experiments on different days (day 1 shown). Data points represent mean ± SEM (two to four replicate wells/concentration). In the antagonist experiments, the cells were preincubated for 5 min with test compound at the indicated concentrations. Currents were elicited with application of 100 μM (−)-nicotine. Agonist data were normalized to the (−)-nicotine maximum current amplitude. Data were fitted to one-site models.