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. 2016 Aug 1;14(6):333–344. doi: 10.1089/adt.2015.688

Fig. 5.

Fig. 5.

Positive modulation of α4β2 and α7 receptors. (A) α4β2-CHO cells were preincubated with LY2087101 alone (addition #1) at the indicated concentrations and then stimulated with 3 μM (−)-nicotine (addition #2). Percent activation was calculated by normalizing against vehicle controls activated by 3 μM (−)-nicotine alone (100% activation). Concentration–response curves (eight point) were obtained in two independent experiments on different days (day 1 illustrated). Data points represent mean ± SEM (two to four replicate wells/concentration). Filled symbols represent addition #1 (LY2087101 in the absence of ligand), open symbols show peak currents recorded during addition #2. Data points were fitted with EC50 = 1.1 μM. (B) α7/RIC-3-CHO cells were preincubated with PNU120596, NS1738, or SB-206553 alone at the indicated concentrations and then stimulated with 20 μM (−)-nicotine. Percent potentiation was normalized against maximal signal [20 μM (−)-nicotine with 10 μM PNU120596]. Data were fitted with EC50 values, 21.7, and 0.6 μM, respectively, for NS 1738 and PNU 120596. The EC50 value for SB-206553 was greater than 50 μM. CHO, Chinese hamster ovary.