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. Author manuscript; available in PMC: 2017 Aug 17.
Published in final edited form as: Neuron. 2016 Aug 17;91(4):777–791. doi: 10.1016/j.neuron.2016.07.005

Figure 4. Impaired Ca2+ influx upon active zone disruption.

Figure 4

A. Sample images of cKOR+E and controlR+E neurons filled via patch pipette with Fluo-5F and Alexa 594 (red, top) and enlarged view of boutons (bottom) analyzed in B.

B. Somatic action potentials (top) and presynaptic Ca2+ transients imaged via Fluo-5F fluorescence (bottom) of the color coded boutons shown in A.

C. Summary plots of single action potential-induced Ca2+ transients in boutons, inset: same plot for dendrites. Data are shown as mean (line) ± SEM (shaded area). ***p < 0.001 for Ca2+ transients during the first 60 ms after the action potential as assessed by two-way ANOVA for genotype and time; interaction n.s. (boutons: controlR+E n = 202 boutons/16 cells/3 independent cultures, cKOR+E n = 157/13/3; dendrites: controlR+E n = 148 dendrites/16 cells/3 independent cultures, cKOR+E n = 100/13/3). All numerical data are in Table S4.