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Bufalin inhibits the Hedgehog signaling pathway in vitro. (A) Western blot analysis was used to measure the expression levels of PTCH1, PTCH2 and Gli1. GAPDH was used as a loading control. (B) Densitometric analysis of three independent experiments with three replicates per experiment. Data are presented as the mean ± standard deviation. PTCH1, Patched 1; PTCH2, Patched 2; Gli1, glioma-associated oncogene 1.
