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. 2016 Jul 27;14(3):2665–2678. doi: 10.3892/mmr.2016.5550

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Copyright: © Powell et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Ectopic expression of tcf21 and tbx18 in the cardiac and pharyngeal arch regions by wtip mRNA overexpression. Ventral view of 48 hpf whole-mount in situ hybridization of (A,B) tcf21 and (C,D) tbx18) in (A,C) control and (B.D) wtip mRNA-injected embryos (92 pg/embryo). The ectopic expression of tcf21 (black arrows in B) and tbx18 (black arrows in D) expression in the heart region compared with control tcf21 (yellow arrowhead in A) and tbx18 (yellow arrowhead in C) expression in the PE region of the heart. tbx18 expression in the pectoral fin (black asterisk in C) was unaffected in wtip mRNA-injected embryos. tcf21, transcription factor 21; tbx18, Tbox 18; wtip, Wilm's tumor 1 interating protein; hpf, hours post fertilization; PE, proepicardial organ.