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. 2016 Jul 8;14(3):1993–1999. doi: 10.3892/mmr.2016.5489

Figure 4.

Figure 4

Immunohistochemical detection of neurons and glial cells during Hip-NSC differentiation. (A) Expression of GFAP (green) and Tuj1 (red) in differentiated Hip-NSC cultured with DMEM/F12 containing 10% fetal bovine serum on day 3. (B) Expression of GFAP (red) and Tuj1 (green) and (C) CNPase (green) and Hoechst (blue) in differentiated Hip-NSCs cultured with DMEM/F12 supplemented with 2% fetal bovine serum and 100 ng/ml fibroblast growth factor-8 on day 3. Magnification, ×200. Hip-NSC, hippocampal neural stem cell; GFAP, glial fibrillary acidic protein; Tuj1, β-Tubulin III; CNPase, 2′,3′-cyclic nucleotide 3′-phosphodiesteras DMEM, Dulbecco's modified Eagle's medium.