Figure 3.

Effect of Ang (1-7) and A779 on the generation of ROS stimulated by H₂O₂. (A) Effects of Ang (1-7) (10⁻⁸ mol/l) and A779 (10⁻⁶ mol/l) on the glucose (16.7 mmol/l)-stimulated insulin release from INS-1 cells in the presence of H₂O₂. ROS levels were elevated markedly (46.8%) following H₂O₂ treatment which was partly reversed by Ang (1-7) (27.1%). This effect was blocked by A779. (B) The effect of Ang (1-7) and A779 on the generation of ROS stimulated by H₂O₂ was tested by flow cytometry. ^*P<0.05; ^***P<0.001. Data are presented as the mean ± standard error of the mean (n=3). Ang (1-7), angiotensin (1-7); ROS, reactive oxygen species; DHE, dihydroethidium.