Figure 5.

Ang (1-7) restored the MMP in the presence of H₂O₂. (A) Flow cytometry using JC-1 in INS-1 cells. (B) JC-1 staining in INS-1 cells. MMP was evaluated by laser confocal microscopy. Ang (1-7) decreased the green fluorescence (column 1) and increased the red fluorescence (column 2) when compared with the Con + H₂O₂ groups. Ang (1-7) restores MMP in the presence of H₂O₂. The staining was quantified and presented as a graph. Ang (1-7) treatment reversed the decreases in the MMP induced by H₂O₂ (15 min at 250 µM H₂O₂), and this effect was blocked by A779. ^*P<0.05 and ^***P<0.001. Data are presented as means ± standard error of the mean (n=3). MMP, mitochondrial membrane potential; Con, control.