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. 2016 Jul 29;14(3):2503–2510. doi: 10.3892/mmr.2016.5578

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Copyright: © Xie et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Erythroid transcription factor mRNA levels in the three groups. (A) Quantitative PCR under normoxia. HIF-1α mRNA levels in the negative control and interference groups were lower than that in the overexpression group while HIF-1α mRNA levels in the interference group was lower than those in the negative control group. GATA-1 mRNA expression in the overexpression group was higher than that in the negative group. GATA-1 mRNA expression in the interference group was lower than that in the overexpression group. GATA-2 mRNA expression in the interference group was higher than that in the negative and overexpression groups. GATA-2 mRNA expression in the overexpression group was lower than that in the interference group. NF-E2-mRNA expression in the interference group was lower than the other groups. Differences were statistically significant (P<0.05). (B) Quantitative PCR under hypoxic condition. HIF-1α mRNA level in the negative control and interference groups was lower than that in the overexpression group. HIF-1α mRNA level in the interference group was lower than that in the negative control group. GATA-1 mRNA in the overexpression group was the highest. GATA-2 mRNA in the interference group was the highest. NF-E2 mRNA level in the interference group was the lowest while NF-E2 mRNA level in the overexpression group was lower than that in the negative control group. Differences were statistically significant (P<0.05). (C) The transcription level of the four transcription factors in the negative control group under normoxia and hypoxia. HIF-1α, GATA-1 and NF-E2 mRNAs in the negative control group under hypoxia were higher than that in normoxia. Differences were statistically significant (P<0.05). (D) The transcription level of the four transcription factors in the overexpression group under normoxia and hypoxia. HIF-1α mRNA level in the overexpression group under hypoxia was lower compared to that under normoxis. GATA-1 and GATA-2 transcription under hypoxia was higher than under normoxia. Differences were statistically significant (P<0.05). (E) Transcription factor mRNA levels in the interference group under normoxia and hypoxia. HIF-1α and GATA-2 mRNA levels under hypoxia were higher than under normoxia. The differences were statistically significant (P<0.05). HIF-1α, hypoxia-inducible factor-1α; NF-E2, nuclear factor-erythroid 2.