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. Author manuscript; available in PMC: 2017 May 28.
Published in final edited form as: J Mater Chem B. 2016 Apr 1;4(20):3584–3593. doi: 10.1039/C5TB02747K

Figure 1.

Figure 1

Osteoblast differentiation of rat bone marrow-derived MSCs increased with substrate modulus. (A) Substrate modulus of 2D PUR films increased with decreasing polyol molecular weight (error bars denote standard deviation). (B) FAK (left) and bright-field (right) images of MSCs cultured on PUR films. (C) Cell area and (D) FAK protein expression increased with substrate modulus. (E) Expression of Runx2 and Osx increased with substrate rigidity. (F) Alp gene expression and (G) ALP enzyme activity increased with substrate rigidity from D4 to D10. (H) Alizarin S staining of mineral nodules (black arrows) formed by MSCs on PUR films at D21 showed increased mineralization on rigid films. * p < 0.05.