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. Author manuscript; available in PMC: 2017 May 28.
Published in final edited form as: J Mater Chem B. 2016 Apr 1;4(20):3584–3593. doi: 10.1039/C5TB02747K

Figure 6.

Figure 6

The role of the BMP signaling pathway in substrate-mediated osteoblast differentiation. (A) Expression of Runx2 and Opn significantly decreased with increasing dorsomorphin dose on rigid well plates. (B) Dorsomorphin (1 μM) reduced Runx2 expression on rigid substrates. (C) Silencing Iβ1 inhibited expression of Runx2 and Opn, which was only partially recovered by treatment with exogenous rhBMP-2 (100 ng/ml). (D) Expression of Alp and Osx significantly increased with both substrate modulus and exogenous rhBMP-2 concentration (increases in Runx2 expression were not significant) (E–H) Mineralization increased significantly with substrate modulus but not rhBMP-2 concentration. * p < 0.05.