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. 2016 Aug 19;12(8):e1006227. doi: 10.1371/journal.pgen.1006227

Fig 5. qRT-PCR analysis of the effect of sig-7(RNAi) on splicing.

Fig 5

qRT-PCR was performed on several strictly embryonic (A), and strictly maternal genes (B) affected by sig-7 RNAi. In order to distinguish unprocessed pre-mRNA from mRNA, primers were designed to amplify either outron, introns, or intron-exon junctions as indicated with green arrows. Other primers were designed to amplify only spliced exon-exons of mRNAs as indicated with red arrow. The relative abundances measured by qRT-PCR of respective RNA are shown for each gene as are the ratios of pre-mRNA/mRNA. For maternally expressed genes, no pre-mRNAs were detected. The expression for each was normalized to 18S RNA and plotted relative to L4440 controls in each experiment. Error bars = S.D. from three technical replicates each of four biological replicates.