Fig 1. Myd88 ^-/- mice fail to mount Th17 and Th1 cells and resistance after vaccination.

Wild type C57BL6 and Myd88 ^-/- mice were s.c. vaccinated with 10⁵ or 10⁶ live B.dermatitidis yeast and followed for survival (A). To circumvent dissemination, mice were vaccinated s.c. with 10⁶ heat-killed yeast and challenged i.t. with 2 x 10³ 26199 yeast. Two weeks post-infection, lung CFU were enumerated (B). Data are the mean ± SEM (n = 8–10 mice/group). Data are representative of three independent experiments. The number of Th17 and Th1 cells recalled to the lung were assessed at day 4 post-infection. Data are the mean ± SEM (n = 4–6 mice/group) (C). The numbers indicate the mean ± SEM of cytokine producing CD4⁺ T cells per mouse. * P <0.05 vs. vaccinated wild-type controls. Dot plots show concatenated samples of 4–6 mice/ group. Data are representative of three independent experiments. (D) Wild-type and Myd88 ^-/- mice were vaccinated s.c. with 10⁷ live H. capsulatum yeast and challenged with 5 x 10⁵ Hc G217B yeast. Two weeks post-infection, lung CFU were enumerated. * P <0.05 vs. vaccinated wild-type controls. (E) The frequencies of Th17 and Th1 cells were enumerated at day 4 post-infection. Data are the mean ± SEM (n = 4–6 mice/group).