Fig 2. Intrinsic MyD88 is dispensable for the development of Th17 and Th1 cells.

Naïve Myd88 ^-/- and Myd88 ^+/+ 1807 T cells (indicated by -/- and +/+) were adoptively transferred into wild type C57BL6 recipient mice prior to s.c. vaccination (Panels A-C). Expansion (A), contraction (B) and migration (C) of transferred 1807 cells and endogenous CD4⁺ T cells was determined by FACS in the skin draining lymph nodes (sdLN) on days 7 and 35 post-vaccination and in the lung at day 4 post-infection. Data are representative of five independent experiments. * P < 0.05 vs. wild type 1807 T cells. (D) Myd88∆T mice that are CD4-Cre⁺ Myd88fl/fl lack MyD88 in αβT cells and CD4-Cre^- Myd88fl/fl controls were vaccinated with 10⁶ heat-killed vaccine yeast and challenged with wild-type yeast. The number of activated (CD44⁺) and cytokine producing CD4⁺ T cells were enumerated at day 4 post-infection. Lung CFU were determined at day 4 and two weeks post-infection. Data from day 4 post-infection are an average of three independent experiments and day 14 post-infection data are representative of two independent experiments. The numbers in the graph indicate the n-fold change in lung CFU vs. unvaccinated control mice.