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. 2016 Aug 19;12(8):e1006269. doi: 10.1371/journal.pgen.1006269

Fig 3. Pollen tube guidance and reception are compromised in the homozygous hap13-1.

Fig 3

(A) Aniline blue staining of wild-type or hap13-1 pistils emasculated and hand-pollinated with wild-type pollen at 4, 9, or 48 hours after pollination (HAP). Arrows indicate the growth front of pollen tubes. (B-E) Close-ups of a wild-type ovule (B) and hap13-1 ovules of different categories, including hap13-1 ovules unable to attract pollen tubes (C), those pollinated and fertilized (D), or those with overgrown pollen tubes (E) at 48 HAP. Frequency of displayed ovules in total ovules examined is shown on top. Arrowheads point at the micropylar region. (F-G) Immunofluorescence staining of a mature wild-type (F) or hap13-1 ovule (G) using an anti-LURE antibody. Bars = 100 μm for 4 HAP images in (A), 200 μm for 9 HAP or 48 HAP in (A), 50 μm for (B-E), 20 μm for (F-G).