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. 2016 Aug 19;12(8):e1006269. doi: 10.1371/journal.pgen.1006269

Fig 5. Dynamic localization of SUB is disrupted in hap13-1 ovules.

Fig 5

(A-E) Representative CLSM of ovules at different developmental stages from the SUBg-YFP transgenic plants. Dotted lines illustrate the shape of either megaspore mother cell (mmc), nucellus (nu), or of developing ovules (B-C). Arrows point at inner integuments (ii), outer integuments (oi), or punctate vesicles (without labeling). Note that SUBg-YFP was asymmetrically distributed in epidermal cell layers, i.e. excluded from the outward plasma membrane in (B-C). (F-J) Representative CLSM of ovules at different developmental stages from the SUBg-YFP;hap13-1 transgenic plants. Dotted lines illustrate the shape of either megaspore mother cell (mmc) or of developing ovules (G-H), and (J). Arrows point at inner integuments (ii), outer integuments (oi), or punctate vesicles (without labeling). Note that SUBg-YFP was more randomly distributed intracellularly and in larger vesicles. Four independent experiments involving 40 ovules at various developmental stages showed similar results. (H) Fluorescence intensity plots along the red lines across a typical outer integument cell in (B), (E), (G), and (J) are shown. The y-axis values represent signal intensity. The arrows indicate the signal accumulation at the plasma membrane. Bars = 10 μm.