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. 2016 Aug 19;11(8):e0159873. doi: 10.1371/journal.pone.0159873

Fig 7. EMSAs with the isolated regulator SenRc.

Fig 7

20 ng of DNA fragments (~200 bp) consisting of the upstream region of the indicated genes were incubated without (lane 1) or with increasing amounts (0.5 μg, lane 2; 1 μg, lane 3 and 2 μg, lane 4) of isolated SenRc in DNA-binding buffer at 30°C for 15 min. The DNA fragment comprising the internal region (~200 bp) of senRc (in-senRc) was used as a control. To amplify DNA fragments, primers listed in S1 Table were used. Analyses were performed with 7% polyacrylamide gels.